FastQCFastQC Report
Thu 20 Feb 2025
HT2WFDRX5_n02_BS_SMART2_A02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT2WFDRX5_n02_BS_SMART2_A02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64613393
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAA10718721.6589006554724652No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATG8035811.243675595243853No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATT7583661.173697843108162No Hit
GCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGAC7045111.0903482502458894No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTT6780941.049463537691017No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAA4640290.7181622546892097No Hit
GTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAAT3660650.5665466291175887No Hit
CTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACT3476330.5380200355675486No Hit
CACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGG2819310.43633523470900215No Hit
GTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGC2618390.4052395143526977No Hit
CGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT2395570.37075440381222513No Hit
TTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCC1990050.3079934217353359No Hit
GTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAA1943130.3007317693407619No Hit
ATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAA1765150.2731863965107048No Hit
GAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGA1659240.2567950579533875No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCAC1441150.22304199378602513No Hit
CCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAA1434120.22195398406023964No Hit
GTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTC1419290.21965879426886No Hit
TCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTA1413940.21883079255720247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1333260.20634421721205695No Hit
GCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTC1218090.18851973924353424No Hit
ACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTA1212640.1876762608643691No Hit
GGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAA1193700.1847449800384264No Hit
GTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAA1190790.18429460901395472No Hit
GTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGG1160950.17967637142968176No Hit
GTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGT1109810.17176160366628634No Hit
CCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTG1093990.1693131948665813No Hit
GTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGC1093210.1691924768600219No Hit
CTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGA1079020.166996337740691No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG1062370.16441947260067274No Hit
GAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTT1059670.16400160257796706No Hit
GGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTC1013780.15689935985872155No Hit
GTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGA982620.15207683026334803No Hit
AGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTC940130.1455007942393615No Hit
GGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTG932770.14436171151080088No Hit
ATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTA915930.14175544070251814No Hit
TTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCC875180.13544869869316412No Hit
GGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTT872980.13510821200799655No Hit
CCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAG835400.129292080358634No Hit
CCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCC820990.12706189257078637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT818210.1266316412140746No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA811460.12558696615731044No Hit
ATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGA807710.1250065911257748No Hit
GTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGG798540.12358738071532631No Hit
GAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAAT741190.11471151189970785No Hit
TCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGC721130.11160689239767985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)