FastQCFastQC Report
Thu 20 Feb 2025
HT2WFDRX5_n01_BS_SMART2_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT2WFDRX5_n01_BS_SMART2_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57694771
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT9287281.609726468972379No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA9210751.5964618353368625No Hit
GGCTTAGACAGCTAGGATGTTGGCTTAGAAGCAGCCATCATTTAAAGAAA1289420.22348992424287462No Hit
CTTCTGATACGTCCAGCAGCCCTCTCGAGCCACCTTCATCCGCTTACAGA790770.13706094786302211No Hit
GTCTAAGCCTTCCCACTTCGTTTCCCACTTAATATAGACTTTGGGACCTT725300.12571329904403294No Hit
GTATTGCTACTGATGCCGCAGCTTCGGTGCTATATTTGAGCCCCGTTACA642600.1113792444032753No Hit
GACTCACCCTGCCCCGATTAACGTTGGACAGGAACCCTTGGTCTTCCGGC606960.10520190815906004No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)