FastQCFastQC Report
Thu 20 Feb 2025
HT2WFDRX5_n01_BS_SMART2_A02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT2WFDRX5_n01_BS_SMART2_A02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64613393
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAA10663941.6504225370117926No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATG7943621.2294076554685807No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATT7671881.1873513591833817No Hit
GCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGAC7109451.1003059381202904No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTT6726671.0410643502346333No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAA4940070.7645582085435445No Hit
GTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAAT3734080.577911146068432No Hit
CTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACT3549730.5493799095181398No Hit
CACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGG2832870.4384338708230351No Hit
GTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGC2622150.40582143705098417No Hit
CGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT2444970.378399877560988No Hit
TTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCC2194620.3396540404556684No Hit
GTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAA1891590.292755094907336No Hit
ATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAA1775640.2748098989322539No Hit
GAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGA1686770.26105578451823447No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCAC1434970.22208553573405437No Hit
CCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAA1421500.22000082862077835No Hit
TCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTA1396290.21609916074210805No Hit
GTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTC1394500.21582212839372172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1262360.19537125994915633No Hit
GCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTC1246320.19288880248093457No Hit
GGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAA1203870.18631895712395108No Hit
GTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGG1169590.1810135555023399No Hit
ACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTA1157200.17909599639814613No Hit
GTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAA1152880.17842740436181706No Hit
GTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGT1112610.17219495035649962No Hit
CTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGA1107440.1713948066463558No Hit
GTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGC1095740.1695840365479646No Hit
GAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTT1024270.15852286228027057No Hit
GGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTC999710.1547217927403998No Hit
CCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTG988480.1529837629792944No Hit
GTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGA973670.1506916685214163No Hit
AGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTC959240.14845838540006714No Hit
ATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTA929620.1438741964843109No Hit
GGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTG875150.13544405569291185No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA869360.1345479566442208No Hit
GGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTT844150.1306462887655505No Hit
CCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCC839120.12986781238991738No Hit
ATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGA821840.12719344424460113No Hit
CCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAG817440.12651247087426598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT783750.12129838159094973No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG775680.12004941452308503No Hit
TTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCC762110.11794923074230138No Hit
CTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGGATTCTC759590.11755921872110942No Hit
GAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAAT735880.11388970085505339No Hit
GTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGG716670.11091663302684011No Hit
TCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGC647420.1001990407778152No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)