FastQCFastQC Report
Mon 23 Dec 2019
HT27VBGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27VBGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48932625
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG782544315.992281223416892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG511530.10453761677408477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGA157750.049.4418331
GATCGGG163900.046.1621481
ATCGGAG75150.045.030552
TCGGAGG95900.043.8785063
AGCGGAA150200.042.3472062
GCGGAAG197500.038.417163
GGGCGTC197250.035.8495189
GAGCGGC245000.035.368429
GATAGGA100350.031.917831
ATCGGGA83200.029.1275652
ATCGGGG124500.027.9994472
CGGAAGG122250.027.384674
GGTCGGA102100.026.6544231
GAGAGTC78750.026.496749
GGGGAAA5628500.026.49080855-59
CGGAAGA1739600.026.4706024
GATCGGA1671300.026.0444411
GGGAAAA5928950.025.70981655-59
GGGGCGT315950.025.5041188
TCGGAAG1668100.025.273923
Produced by FastQC (version 0.11.5)