FastQCFastQC Report
Mon 23 Dec 2019
HT27VBGXC_n02_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27VBGXC_n02_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52971665
Sequences flagged as poor quality0
Sequence length151
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3674740.6937180471861701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA827350.014.81322655-59
AAAGGGG1061850.013.72709470-74
GGGGAAA1060650.013.33638655-59
AAGGGGG903950.012.80007370-74
AAAAGGG1648050.09.3349470-74
GGGAAAA1654450.09.17874655-59
AGGGGGG1078400.09.03286675-79
GAACCCT224300.08.2642984
GGGGGGA975850.07.880434555-59
TGGTCGC306550.07.2322216145
TCGCCGT276350.07.2098274145
ATCTCGG437950.07.1964173145
CTCGGTG379650.07.080145145
TGAACCC263250.07.06853963
CTGAACC267550.06.95454172
TCTCGGT422000.06.9533515145
GGTCGCC292500.06.836516145
CGGTGGT391950.06.7840185145
GATCTCG481100.06.746745145
TCGGTGG404100.06.723479145
Produced by FastQC (version 0.11.5)