FastQCFastQC Report
Mon 23 Dec 2019
HT27VBGXC_n02_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27VBGXC_n02_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53599317
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2577440.48087179916863493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG668950.011.53200770-74
AAAAGGG738600.010.79426270-74
AAGGGGG716900.08.49762470-74
AAAAAGG1062300.07.188152370-74
GGGGGAA817300.05.658715255-59
AGGGGGG1155350.05.65284675-79
GGGGAAA816450.05.433543755-59
CGTGTAG367650.05.0056534145
Produced by FastQC (version 0.11.5)