FastQCFastQC Report
Mon 23 Dec 2019
HT27VBGXC_n02_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27VBGXC_n02_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43487825
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2458290.5652823520146155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG609250.014.72703570-74
AAAAGGG666250.013.76983970-74
AAGGGGG671850.010.62252570-74
AAAAAGG960600.08.97336870-74
GGGGGAA733300.07.1261355-59
GGGGAAA714050.07.088594455-59
AGGGGGG1101950.06.59956775-79
GGGAAAA805600.06.084886655-59
AAAAAAG1399900.05.34267870-74
Produced by FastQC (version 0.11.5)