FastQCFastQC Report
Mon 23 Dec 2019
HT27VBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27VBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48932625
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11123122.273150071143741No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC5574301.1391786154942638TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC4441730.9077236301956007TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGTAATCTCGTATGC3249340.6640436722943026TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATAATCTCGTATGC2056720.4203167109878123TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1902260.3887508589616846TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATAATCTCGTATGC1863160.38076028007898616TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTCATCTCGTATGC1243690.25416376088550324TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTAATCTCGTATGC1137820.23252788911283626TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGTCATCTCGTATGC1083660.22145960900319572TruSeq Adapter, Index 12 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGTAATCTCGTATGC905490.18504831898963114TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATCATCTCGTATGC883680.18059117000160935TruSeq Adapter, Index 7 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGG753760.1540403769468734No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGTCATCTCGTATGC663420.1355782568378459TruSeq Adapter, Index 12 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCATCTCGTATGC579750.11847923547939643TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATCATCTCGTATGCC520930.10645862550803273TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTATAATCTCGTATGC519940.1062563065030744TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG9020450.0116.212483
GATCGGA8759750.0116.0657351
AGAGCAC8883650.0115.92088
ATCGGAA9137100.0115.672272
CGGAAGA9260050.0113.2613454
GAGCACA9220050.0111.2619559
AAGAGCA9405850.0110.6762167
GAAGAGC9574550.0108.587946
GGAAGAG9624200.0108.384645
TATCGGA179950.0107.437211
AATCGGA207500.075.670031
GAATCGG105600.056.01571
GATCAAG268250.053.371681
GATAGGA86450.048.886291
ATCGGGA102000.044.074272
ACGGAAG113400.043.8502543
GATCCGA58550.042.095491
GGATCGG140000.042.0447271
CGGAAGG114450.041.55054
CGGAAAG125450.040.618954
Produced by FastQC (version 0.11.5)