FastQCFastQC Report
Mon 23 Dec 2019
HT27VBGXC_n01_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27VBGXC_n01_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52289985
Sequences flagged as poor quality0
Sequence length151
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2921518655.871475197401566TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG14974172.8636783888922515TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGTAATCTCGTATGC6493941.241908942984015TruSeq Adapter, Index 12 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGT4300210.8223773634664459TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCGGTCACCTTGTAATCTCGTATGC2596080.49647748034351125TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGTAATCTCGTATGC2301020.44004984893378724TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTCATCTCGTATGC1755270.33567995860010286TruSeq Adapter, Index 12 (98% over 50bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1685370.3223121980241532TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCCGTCACCTTGTAATCTCGTATGC1622770.31034049828088495TruSeq Adapter, Index 12 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGCCGT1525630.29176332714572395TruSeq Adapter, Index 12 (98% over 50bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1350190.2582119692709799TruSeq Adapter, Index 12 (97% over 49bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1317720.25200236718369684TruSeq Adapter, Index 12 (100% over 49bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1287130.24615229857113174TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATAATCTCGTATGC1054910.20174226479506546TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTAATCTCGTATGC966020.18474283364204447TruSeq Adapter, Index 7 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC923350.1765825712132065TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC920140.17596868692924658TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGTAATCTCGTATGC897870.17170974518351842TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC838080.16027543324022755TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTATAATCTCGTATGC816950.15623450647384965TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTAATCTCGTATGC814680.15580038892724868TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGTCATCTCGTATGC811590.15520945358848354TruSeq Adapter, Index 12 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATGC726570.13895012591799366TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGTCATCTCGTATGC685240.13104612671049726TruSeq Adapter, Index 12 (97% over 38bp)
GATAGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC653570.12498951759117927TruSeq Adapter, Index 12 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC630460.12056993322908775TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACTTCTGAACTCCAGTCACCTTGTAATCTCGTATGC618130.11821192911032581TruSeq Adapter, Index 12 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG615510.11771087714024014No Hit
GATCTGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC604660.11563591001221361TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG598590.1144750758677785TruSeq Adapter, Index 12 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTAGTATGC578230.11058140483306698TruSeq Adapter, Index 12 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTTTG556040.10633776238413532TruSeq Adapter, Index 12 (97% over 49bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC543790.1039950575621699TruSeq Adapter, Index 12 (98% over 50bp)
GATCAGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC539180.10311343558427105TruSeq Adapter, Index 12 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGA305600.0134.949391
GAATCGG246750.0126.350221
GATCGGA42607600.0123.5743941
ATCGGAA43538950.0122.832892
TCGGAAG43518200.0122.767353
CGGAAGA44676750.0119.7756964
GAAGAGC44833750.0119.485346
AGAGCAC44700650.0119.481938
GGAAGAG44798450.0119.424495
GAGCACA44654250.0119.407929
AAGAGCA44857300.0119.305447
CATCGGA155800.0116.667951
GGATCGG260900.0106.602941
GACGGAA79050.097.681521
AGCGGAA111950.096.888492
GAGCGGA116000.094.755811
GTTCGGA54050.094.705171
TCGGAAA178250.088.713733
GAACGGA174400.087.470971
CGGAAGG158550.085.6060944
Produced by FastQC (version 0.11.5)