FastQCFastQC Report
Mon 23 Dec 2019
HT27VBGXC_n01_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27VBGXC_n01_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65997220
Sequences flagged as poor quality0
Sequence length151
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2630631039.85972439445177TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATAATCTCGTATGC6157040.932924144380627TruSeq Adapter, Index 7 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG4423170.6702055025954123TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC3028190.4588359933948733TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGGATGC2413650.3657199500221373TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATCATCTCGTATGC2384290.3612712777901857TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTCATCTCGTATGC2287790.3466494497798544TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATAATCTCGTATGC2096040.31759519567642397TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATTC1671360.25324703070826315TruSeq Adapter, Index 7 (98% over 50bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1366920.20711781496250906TruSeq Adapter, Index 7 (98% over 50bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1340280.20308128130245487TruSeq Adapter, Index 7 (100% over 49bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT1329430.20143727266087874TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATATCGTATGC1273910.19302479710509018TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTGC1162780.17618620905547233TruSeq Adapter, Index 7 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1082920.164085699367337TruSeq Adapter, Index 7 (100% over 50bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1061230.1607991972995226TruSeq Adapter, Index 7 (97% over 49bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1059690.16056585413749244TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGTAATCTCGTATGC1023900.15514289844329807TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTAATCTCGTATGC984860.14922749776429975TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAAGC889910.13484052813133643TruSeq Adapter, Index 7 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG859690.13026154738032905No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTATGC826840.12528406499546496TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCATCTCGTATGC813880.12332034591760077TruSeq Adapter, Index 7 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTG811300.12292942036043336TruSeq Adapter, Index 7 (97% over 49bp)
GATCTGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC780000.11818679635293729TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGG750230.11367600029213351TruSeq Adapter, Index 7 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGTCATCTCGTATGC661000.10015573383242507TruSeq Adapter, Index 12 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGA370450.0102.928631
GAATCGG430250.0101.059171
GATCGGA54944000.090.357231
TCGGAAG55772750.090.187893
ATCGGAA55948750.090.0633242
CGGAAGA56329550.089.344164
AGAGCAC56483100.089.066828
GAGCACA56372750.089.0610059
GGAAGAG56533350.089.056015
GAAGAGC56647900.089.032736
AAGAGCA56669050.088.969317
GGTGACT210700.083.68771
CGGTGGT647450.080.841321
GTGACTG195350.080.094182
TGACTGG194300.075.116253
GGATCGG696250.074.008851
GGTGGTC507250.072.742
GGTGGTA214050.069.777422
GTGGTAG213050.069.1520543
AATCGGA671800.064.970972
Produced by FastQC (version 0.11.5)