FastQCFastQC Report
Mon 23 Dec 2019
HT27VBGXC_n01_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27VBGXC_n01_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52971665
Sequences flagged as poor quality0
Sequence length151
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC3377990.6376975313122591TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC2206000.030.2887468
GAGCACA2215400.030.0851029
GAAGAGC2464300.027.1680626
CGGAAGA2534550.026.326244
TCGGAAG2562150.025.9888613
AAGAGCA2671250.025.2141937
GGAAGAG2716000.024.6796345
GATCGGA2656250.024.6641851
ATCGGAA2732400.024.4146582
AAGGGGG703000.019.53880170-74
TGCCGTC708550.019.41038745-49
GCCGTCT685000.019.04259945-49
CTGCTTG720600.018.73567655-59
ATGCCGT746350.018.23692545-49
TATGCCG748650.018.15378445-49
CCGTCTT744300.017.9754650-54
AGGGGGG722200.017.4653670-74
GCTTGAA761900.017.25190755-59
TGCTTGA808200.016.98122655-59
CGTATGC799200.016.47936440-44
Produced by FastQC (version 0.11.5)