FastQCFastQC Report
Mon 23 Dec 2019
HT27VBGXC_n01_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27VBGXC_n01_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13734524
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC356157525.931550303454276TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC1188050.8650099559329468TruSeq Adapter, Index 5 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCCGT964260.7020701991565197TruSeq Adapter, Index 5 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT902150.6568483916879828TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTGATCTCGTATGC495000.3604056463842504TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTGATCTCGTATGC433520.31564253701111156TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAAGTGATCTCGTATGC390980.28466949418851356TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGCTCTCGTATGC323460.23550870783727199TruSeq Adapter, Index 5 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGCCGT226700.1650585051218375TruSeq Adapter, Index 5 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG209420.15247707164806001No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTCATCTCGTATGC190930.13901464659423218TruSeq Adapter, Index 5 (98% over 50bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC171950.12519545635509466TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTTATCTCGTATGC159550.11616711289011544TruSeq Adapter, Index 5 (98% over 50bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC144980.10555880931876489TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATTC142300.10360752218278552TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4887600.0138.018021
TCGGAAG4993900.0136.790473
ATCGGAA5004650.0136.640082
CGGAAGA5556300.0122.999564
AGAGCAC5577000.0121.8451468
GGAAGAG5595750.0121.8240365
GAAGAGC5598250.0121.737696
GAGCACA5584900.0121.3742459
AAGAGCA5640000.0120.7780467
GAGCGGA15200.0110.189311
GAATCGG39900.097.9460451
GCTCGGA13950.092.515991
GGATCGG33350.085.006471
GCGGAAG23550.080.972183
GAGGCAC24500.079.899969
GATCGGC4800.078.5476461
GATCCGG10700.077.926481
CGGAAGG30650.077.591554
CTCGGAA17050.074.84442
CGGAAAG25800.074.191694
Produced by FastQC (version 0.11.5)