FastQCFastQC Report
Mon 23 Dec 2019
HT27VBGXC_n01_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27VBGXC_n01_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53599317
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG607700.06.47841453
AGAGCAC642600.06.47612958
ATCGGAA613100.06.32674932
GATCGGA627600.06.0072891
TCGTATG154350.05.9465340-44
GAGCACA728700.05.81045969
AAGGGGG294200.05.8009670-74
CTCGTAT151850.05.519243240-44
CGGAAGA765150.05.3442984
AAGAGCA818400.05.3241817
AGGGGGG336550.05.03221770-74
Produced by FastQC (version 0.11.5)