FastQCFastQC Report
Mon 23 Dec 2019
HT27VBGXC_n01_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27VBGXC_n01_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43487825
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG409750.018.2783743
ATCGGAA445950.016.7621122
AGAGCAC472050.016.0806628
GATCGGA464350.015.70756151
GAGCACA512500.014.9530189
CGGAAGA573450.013.4019214
AAGAGCA578750.013.2537557
GGAAGAG606050.012.72895
GAAGAGC723300.010.8259126
CTCGTAT148550.09.65361740-44
TCGTATG160050.09.05057740-44
AAGGGGG219800.08.23950370-74
AGGGGGG217600.07.809711570-74
AAAGGGG241650.07.332476670-74
TCTCGTA200000.07.22097440-44
GTATCTC192150.06.595353635-39
CGTATGC228850.06.31066240-44
TATCTCG215600.06.22772335-39
AAAAGGG330550.05.641181570-74
GTCTTCT281500.05.614548750-54
Produced by FastQC (version 0.11.5)