FastQCFastQC Report
Tue 26 Nov 2019
HT27GBGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27GBGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26373314
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7364552.7924249489464996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC45050.029.7063791
TCCGGCT13400.027.6135232
CGCGTAA60750.026.6381171
CAGACGT35750.024.6834813
TTTCGTT75600.024.6236048
CGTAACT67650.022.8622683
GCAGACG34500.022.8255392
GTAACTA70000.022.7091754
TCCTGGC18450.022.1340587
CTCCTGG27800.021.8587686
ACTCCTG29100.021.8578435
TCGTGCG12850.020.6939747
CGATCGA21150.020.6410624
CCTGGTG30400.020.1513188
GATCCTG20550.020.095695
CTCGGCT19850.020.0823351
CGACTTT72350.020.0039564
TGATCCT21000.019.884654
ACTAGTT77700.019.8619237
CGGCTAT28950.019.8488339
Produced by FastQC (version 0.11.5)