FastQCFastQC Report
Tue 26 Nov 2019
HT27GBGXC_n02_SEGAL_PACT_M16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27GBGXC_n02_SEGAL_PACT_M16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67737942
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAG2930820.43267036367889655No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC2842440.41962302309095834Illumina Single End PCR Primer 1 (97% over 35bp)
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT2808910.41467306461716835No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA2645100.39049016281008364No Hit
GCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCC2633870.38883230317212764No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT2591520.38258026793905253No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG2421760.3575189810165771No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2162030.319175625382891No Hit
GGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGAC2038130.300884547097696No Hit
CCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCA1965550.29016972496743404No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1936620.28589885414587884No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT1931710.28517400189099334No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG1927030.2844831040187197No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1920850.2835707645207172No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1881330.277736515821517No Hit
GGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACT1877090.27711057415945706No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG1872240.27639457956960073No Hit
GGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGA1796940.2652782099580173No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG1708930.25228549163775893No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAG1617440.23877902874580983No Hit
GGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGC1611860.23795526589809887No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC1499580.22137962207354925No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG1464430.21619050664397213No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAA1448680.2138653695738202No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT1420520.2097081721201391No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG1324620.1955506708485475No Hit
GCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCT1295330.19122665403681735No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGA1222110.18041735014624447No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1179760.17416531491316933No Hit
GCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCT1135930.16769479060937517No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA1119950.1653356991566115No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT1118630.1651408305259702No Hit
TGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA1098080.1621070802534863No Hit
GCCGGGCGCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCA1069030.1578184941018728No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1040720.15363915248561877No Hit
GGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCG1026650.15156203003628305No Hit
CCGGGCGCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCAC1006800.14863161918913922No Hit
GTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCT998060.147341352649893No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC992650.14654268651976465No Hit
CGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTAC966570.14269255478709406No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCCGCTCGTGGGGGG964010.14231462774585033No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC938210.13850583178331577No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC938170.13849992667329633No Hit
GAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACT928640.13709303421116634No Hit
GGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAG906530.13382898464792448No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG901320.13305984406789328No Hit
CCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTG895700.13223017611016288No Hit
GGCCAACCGAGGCTCCTTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGA889480.131311931502141No Hit
GCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCT889050.13124845156943207No Hit
GCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAG861520.12718425959855703No Hit
CGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGCCAAGGG848700.12529167183732862No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG848660.1252857667273092No Hit
GCTCCTTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAG840370.12406193267578162No Hit
GGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGAT829620.1224749343580589No Hit
GGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTC824740.12175451093568801No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG823970.12164083756781392No Hit
GCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGA799230.11798852702079433No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT796730.11761945764457976No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA796480.11758255070695829No Hit
CGGCGCGCCGGGCTCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAG792210.11695218021238378No Hit
CGGCCCCCGGCGCGCCGGGCTCGGGTCTTCCCGGAGTCGGGTTGCTTGGG788980.11647534257831452No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA787200.11621256518244974No Hit
GCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG787160.1162066600724303No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC785990.11603393560436188No Hit
GGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATT773140.1141369190106189No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG771500.11389480949982211No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA756520.1116833457975443No Hit
GGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGA755910.11159329286974794No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT754940.11145009395177669No Hit
GGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAG743530.10976566131873329No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT728370.10752762462136803No Hit
CGGGCGCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACC728090.10748628885123199No Hit
GGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTA726160.10720136729279434No Hit
TCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGT717160.10587271753842181No Hit
CGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCT712220.1051434364510218No Hit
GCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGC710580.10490132694022503No Hit
CATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAA698350.10309583955178325No Hit
GCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAA695890.1027326752855881No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC694650.10254961687498566No Hit
AAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCT689600.1018040967350322No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC688480.10163875365448805No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG681750.10064521889371837No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC679610.1003292955076787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA579500.048.3947071
CCTGGCC112600.046.726071
CTCACCC443850.045.0393142
CGTATCA391650.044.32226662-63
TCCGGCT92100.043.029522
GTATCAT396900.042.6885362-63
GCGTAAC668900.042.317122
GTGGTCG437100.041.39782754-55
CGCCGTA429700.041.36150458-59
ATCATTA432450.040.5524164-65
GGCGCTT53550.040.237972
TCGGTGG439450.040.0402750-51
CTCGGTG403850.039.9817150-51
TCTCGGT414800.038.99579248-49
CGTAACT734300.038.70633
GGCCTTA142050.038.0232624
TATCATT460850.037.38846664-65
GGTCGCC485450.037.18367456-57
TAGATCT441200.036.62288344-45
GCCTTAT147950.036.451445
Produced by FastQC (version 0.11.5)