FastQCFastQC Report
Tue 26 Nov 2019
HT27GBGXC_n02_SEGAL_PACT_M15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27GBGXC_n02_SEGAL_PACT_M15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48824028
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAG1773280.36319821871313035No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT1532980.3139806490361672No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG1294500.265135846636824No Hit
CCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCA1181650.2420222272525323No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG1140990.2336943604898801No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT1139660.23342195363315782No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA1085480.22232495852247178No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1028980.21075278754141302No Hit
GCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCC1018850.20867798945224267No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG974730.19964145522774154No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA861430.1764356681099724No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA852200.17454520548775698No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT828850.16976272420620436No Hit
GGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGAC793040.16242822079325367No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCCGCTCGTGGGGGG791540.16212099501499547No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC781300.1600236670354195No Hit
TGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA769730.1576539321991213No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT739390.15143977879088552No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG738300.15121652805868455No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAG738000.15115508290303292No Hit
GGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGC700810.14353793177408466No Hit
GGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGA685270.14035507271132977No Hit
GGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACT664690.13613993503362729No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG655890.1343375438011792No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC623250.12765231086628084No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC605680.12405367291694983No Hit
GAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACT593250.12150779530111691No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGA562800.1152711120024755No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAA561310.11496593439607236No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG558820.11445593960416375No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT555440.11376365751715528No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG544970.11161922158491307No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT543300.11127717688511893No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT536750.10993562432005816No Hit
CCGGGCGCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCAC522640.10704565383257605No Hit
CCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCT516670.1058228952351084No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG511950.10485615811952262No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA493160.10100764320387495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCT70000.048.6119272
CTCCGAC312750.041.75331
GCGTAAC292150.039.4749952
TTCGTTC515800.035.0807239
CGCGTAA360200.033.141871
GGCCTTA86250.032.8516654
GGCGCTT36950.032.7527242
GCCTTAT92750.030.4025785
GTAACTA392850.029.828854
CGCTAGG24650.029.2663694
TTTCGTT638650.028.6365878
GGCAGAC172550.028.4826761
CGATCGA131650.028.4805534
CAGACGT209750.027.5569423
CGTAACT422000.027.3936863
TGGCCTT100800.027.3527683
GGTTATT95900.027.216636-37
GGTTTTT515100.027.08302736-37
GATCCTG45550.026.5760025
CTCACCC290150.026.4980662
Produced by FastQC (version 0.11.5)