FastQCFastQC Report
Tue 26 Nov 2019
HT27GBGXC_n02_PACT_MOCK.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27GBGXC_n02_PACT_MOCK.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64773848
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGCGATATCAGCCGAAGAAGGACGCGGTAAGCTGCGATAAGCTACGG2052790.3169164814787598No Hit
GTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATGCAA1840540.28414862739048635No Hit
CCTCGATCGATTAGTACCAGTCAGCTCCAAACCTCACGGCTCTTCCACAC1571180.2425639433988853No Hit
CTAGGAGATACACCTGTACCCATGCCGAACACAGAAGTTAAGCCCTAGAA1089130.16814347666978194No Hit
CGGATGCCTTGGCTCACGGAGGCGATGAAGGACGTGATAAGCTGCGATAA979910.15128173333163716No Hit
AGGAGATACACCTGTACCCATGCCGAACACAGAAGTTAAGCCCTAGAACG919190.14190757973804488No Hit
GGATGCCTTGGCGATATCAGCCGAAGAAGGACGCGGTAAGCTGCGATAAG887200.1369688581725143No Hit
GGCGGATGCCTTGGCTCACGGAGGCGATGAAGGACGTGATAAGCTGCGAT858760.13257819729962625No Hit
CTGCGATAAGCCTTGGGTAGGTGCAAATAACCTTTGATCCAAGGATTTCC853260.13172908918426462No Hit
TGATAAGCTGCGATAAGCCTTGGGTAGGTGCAAATAACCTTTGATCCAAG825620.1274619349463382No Hit
GTCGATATCAGTATTTTGGCTTGGAGGGTGGTCCCCCCTGCTTCCCACAA805220.12431251575481513No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG771410.11909281659474669No Hit
CCTGGACCGATTCTCTGCGCCTCATATTGCTATGAGGACCCCTTATCCCG714940.1103747919993884No Hit
CGCGAAACCAAGTGATCTACACTTGCCCAGGTTGAAGTCCGGGTAACACC698770.10787841414022523No Hit
CCCGGACGTAAGGGCCGTGCTGATTTGACGTCATCCCCACCTTCCTCACA696510.10752950789645846No Hit
CTGGATTCTTCTCCTCTCGGGGACGGACCTTAGCACCCGCCCCCTTACTG688100.1062311443964237No Hit
GGTGGATGCCTTGGCGATATCAGCCGAAGAAGGACGCGGTAAGCTGCGAT687240.10609837476383988No Hit
CGACGATATGCCTTGGGTAGCTGTAAGTAAGCGATGATCCAGGGATTTCC682280.10533263362707741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATC237050.072.896162
TCGATCG254800.068.5953453
CGATCGA271050.065.1921164
CGATTAG289200.061.382488
TGATCCT389350.058.196074
ATCGATT327950.054.4497346
GCGTACG77550.051.1009062
GATCGAT384550.048.232085
TCCTGGC484000.047.7622877
GATTAGT381700.046.5084659
AGGATCC59300.043.1346633
CGTACGG93700.041.9356273
TAGGAGA286950.041.8775632
CTGGCTC564800.040.0330169
CCTGGCT572100.039.512868
CGCCCTC36200.038.5640031
GTCCCAC74800.037.776258
GATCCAC73700.035.7495735
GGATCCA75350.034.8331954
CTTTGAC184900.032.97076
Produced by FastQC (version 0.11.5)