FastQCFastQC Report
Tue 26 Nov 2019
HT27GBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27GBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26373314
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8244753.126171401895113No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT327080.12401930223861894TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC575200.043.144368
GAATCGG23300.042.3159371
AATCGGA26800.037.667612
TCGGAAG710150.035.6995743
GAGCACA706550.035.3650179
CGGAAGA740550.034.9786224
TATCTCG114400.034.76195540-41
GATCGGA727100.034.708871
ATGCCGT275200.034.11244248-49
GCTTGAA264850.034.026760-61
AAAGGGG224250.033.26291372-73
CACATTC100600.033.07844530-31
CTCGTAT256800.032.9218144-45
CGTATCT74900.032.6975638-39
AAAAGGG220100.032.61448372-73
AAGAGCA790250.032.5079157
ATCGGAA790450.032.4913672
CAGTCAC305100.032.254826-27
TCGTATC80350.031.9241438-39
AGGGGGG245450.031.56671174-75
Produced by FastQC (version 0.11.5)