FastQCFastQC Report
Tue 26 Nov 2019
HT27GBGXC_n01_SEGAL_PACT_NEGATIVE2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27GBGXC_n01_SEGAL_PACT_NEGATIVE2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9487022
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT230932724.341958941383293TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGTCGTAT784240.8266450736595741TruSeq Adapter, Index 13 (97% over 38bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTA524890.5532716167412703TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGCGTAT373910.3941278938743896TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT305660.32218751047483607TruSeq Adapter, Index 13 (97% over 38bp)
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT282270.2975327768819341No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTA239820.2527874395147392TruSeq Adapter, Index 13 (97% over 38bp)
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA229610.24202536897247628No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG219710.23159006061122234No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA208870.22016392499142512No Hit
CGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAG195000.20554395257015318No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT174180.1835981828649707No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG173630.18301844351156768No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG167740.17680996207239744No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAA167160.17619860057244519No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC166410.1754080469087138No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTTT161630.17036958489186596TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAA154420.16276972900452852TruSeq Adapter, Index 13 (97% over 38bp)
GGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGC148840.1568880097463672No Hit
CCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCA146170.15407363870348356No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTAGTAT138030.14549349627311922TruSeq Adapter, Index 13 (97% over 38bp)
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT135110.1424156073423251No Hit
GAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACT130150.13718741244618174No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG130120.13715579029963249No Hit
GCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCT129490.13649172522209815No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT125410.13219111329139957No Hit
TGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA119950.12643588261943528No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC113860.12001658686993663No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC112150.11821412451662915No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCCTAT110740.1167278836288142TruSeq Adapter, Index 13 (97% over 38bp)
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCCGCTCGTGGGGGG107410.11321782536184695No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAG106230.11197402093090962No Hit
TAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTT106140.11187915449126186No Hit
GCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAA105630.11134157799992453No Hit
CGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGG105510.11121508941372751No Hit
CCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCT103010.10857991053462299No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG102490.10803179332776924No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAAGCTCGTAT101100.10656663387098712TruSeq Adapter, Index 13 (97% over 38bp)
TCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGT100820.10627149383652741No Hit
GGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACT100500.10593419094000205No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT98140.10344658207812737No Hit
CGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGCCAAGGG98030.10333063420744676No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT97350.10261386555233035No Hit
GTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCT96930.10217115550064076No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA96600.10182331188859897No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT95660.10083248463005566No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG95320.10047410030249745No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGA73800.085.083782
GAATCGG73800.085.044071
GAGCACA3440750.077.9242559
AGAGCAC3487750.076.9327248
AAGAGCA3542150.075.904067
GAAGAGC3591550.074.783846
GATCGGA3631200.073.979411
TCGGAAG3643850.073.8549043
CGGAAGA3656800.073.663114
GGAAGAG3657650.073.579115
ATCGGAA3752700.071.7950742
CATCGGA8850.068.717011
ATCGGGA11800.062.793432
ATCGCAA7650.047.1871532
TGCTTGA2540950.046.41884260-61
TGCCGTC2667550.046.3878550-51
CTTGAAA2636500.046.29746662-63
CTCGTAT2617400.046.16376544-45
TATGCCG2753900.046.03321548-49
CGTATGC2749550.046.0229946-47
Produced by FastQC (version 0.11.5)