FastQCFastQC Report
Tue 26 Nov 2019
HT27GBGXC_n01_SEGAL_PACT_M20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27GBGXC_n01_SEGAL_PACT_M20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70289824
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT4380410.6231926260051526TruSeq Adapter, Index 7 (97% over 38bp)
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT3432830.4883822158951486No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA3071530.4369807498735522No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG2785820.39633332984302255No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA2474420.3520310422174339No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG2401830.3417038005387522No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC2213490.3149090258072065No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2180760.3102525907590834No Hit
CGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAG2020290.28742282808959657No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG2019160.2872620651319315No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT1879710.26742277801122394No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT1870290.26608261247033427No Hit
GGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGC1792540.25502126737434994No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG1670670.23768305352421995No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAA1595730.2270214818008365No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAG1492150.2122853515752152No Hit
GAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACT1484170.21115005210427049No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCCGCTCGTGGGGGG1477810.21024522696201373No Hit
CCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCA1463190.2081652672796563No Hit
GCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCT1450930.20642106032304192No Hit
TGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA1383460.19682223133749774No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG1369670.19486035418156689No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1324980.18850239260806798No Hit
CGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGG1307820.18606107194122437No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT1300760.18505665912607777No Hit
CGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTAC1300200.18497698898776585No Hit
CGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGCCAAGGG1268380.18045001791439966No Hit
TCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGT1266440.18017401779239053No Hit
CCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCT1261960.17953665668589525No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC1260450.17932183184866135No Hit
TAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTT1198740.1705424671428968No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG1196590.17023659071902073No Hit
GTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCT1187680.16896898191123655No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT1151400.16380749509345763No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC1130970.1609009577261141No Hit
GGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACT1127930.16046846268956372No Hit
GCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAA1122270.15966322522019688No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG1070330.15227381989176697No Hit
GGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGAC1056200.15026357158043246No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC1054610.1500373652948683No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT1045570.1487512616335474No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1040290.14800008604374937No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC1035060.14725602385915776No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT1011150.14385439348944734No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGA1002250.14258820736270444No Hit
GCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCT998500.1420547019722229No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC994830.1415325780300716No Hit
GCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCC979660.13937437089044355No Hit
GCTCCTTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAG959720.13653754489412293No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA956080.1360196889950955No Hit
CTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGC943950.13429397689201783No Hit
GGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCG935960.1331572547400318No Hit
GCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG933560.1328158112901236No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG916080.1303289648299589No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG909300.12936438708396822No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA903880.1285932939595922No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA892490.12697286025356957No Hit
CGGGGACCCGGCTATCCGGGGCCAACCGAGGCTCCTTCGGCGCTGCCGTA870570.12385434341107468No Hit
GGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATT853000.12135469282153843No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT852730.12131628043342377No Hit
GGCCAACCGAGGCTCCTTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGA837510.11915095988858929No Hit
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCA833500.11858046479103433No Hit
TGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGC827380.11770978399376843No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA827190.11768275305398403No Hit
GGCGTACGGAAGACCCACTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC826240.11754759835506204No Hit
GGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTC825080.11738256735427308No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT814340.1158546079159339No Hit
CTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCTCCCGA809640.11518594782653033No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC805630.11461545272897539No Hit
GGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAG803470.11430815362405801No Hit
GCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG789420.11230928676105377No Hit
CTTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGC780600.11105448208264115No Hit
CATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAA775080.1102691621478523No Hit
GGAAGACCCACTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGAT771100.1097029350934212No Hit
GCCGGGCGCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCA767090.10913243999586626No Hit
TGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCC766600.10906272862484334No Hit
CCGGGCGCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCAC762100.10842252215626545No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT756410.10761301664377478No Hit
CGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGTT756030.10755895476420597No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA755180.10743802687569683No Hit
CCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTG752970.10712361436557302No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG752330.10703256277893083No Hit
GGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTA747160.10629703668058695No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG737670.10494691237240827No Hit
TCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGC731900.10412602541158732No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCTCCCGACGTACGCAGT731200.10402643773869742No Hit
CAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTA726780.10339761271844983No Hit
GGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGCTGAGGAGCCAAT726760.10339476735636727No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAG724480.10307039607895448No Hit
CGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAG722120.1027346433532114No Hit
ACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTC717270.1020446430481886No Hit
GGGGACCCGGCTATCCGGGGCCAACCGAGGCTCCTTCGGCGCTGCCGTAT715980.10186111719386294No Hit
GCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCT715160.10174445734847763No Hit
TATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATC714590.10166336452912443No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT710410.10106868385386766No Hit
CCGACCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGT709520.10094206524119337No Hit
CTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTT708870.1008495909735099No Hit
GCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAG703520.10008845661642288No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA573250.047.3729361
GGCGCTT85800.045.504812
CTCACCC506550.043.461052
GCGTAAC633800.043.4359132
TCGTGCG88850.040.0912067
GAATCGG40250.040.0143661
AATCGGA40150.039.9856262
CTGCTTG611700.038.53876558-59
CGTAACT718300.038.411743
TGCCGTC672550.038.1145150-51
AAAAGGG502650.037.3942572-73
CCTGGCC110250.036.4564131
GCTTGAA659950.036.26742660-61
GCCGTCT661350.036.22458350-51
TGCTTGA715400.035.40818460-61
CCCGCTA40150.035.3719022
ATGCCGT686350.035.20631448-49
TCGAAGG95500.034.9123427
CTTGAAA738700.034.77028762-63
GTGCGTC104000.034.2052769
Produced by FastQC (version 0.11.5)