FastQCFastQC Report
Tue 26 Nov 2019
HT27GBGXC_n01_SEGAL_PACT_M16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27GBGXC_n01_SEGAL_PACT_M16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67737942
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT26696973.941213625887837TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTA3342420.4934339457788664TruSeq Adapter, Index 7 (97% over 35bp)
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT3186180.4703685860429595No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG3105450.45845059774623803No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA2780020.4104080989056325No Hit
CGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAG2233870.32978120297779345No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA2166630.31985471303512586No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2151430.3176107712277412No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT2048700.3024449724203313No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAA1983360.2927989752035868No Hit
CCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCA1948260.287617241161534No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG1924910.2841701331876897No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT1839370.27154205541113136No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG1811880.2674837685502757No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG1782470.26314203640848727No Hit
GGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGC1760260.2598632240701969No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC1681950.24830249492965112No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1616580.23865206888039203No Hit
GGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACT1587410.2343457673987202No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT1530580.22595608233861017No Hit
GGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGAC1408390.20791744750674593No Hit
GCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCC1389710.2051597611276705No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1369560.2021850619553809No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1330140.1963655760312293No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC1296800.19144366683003153No Hit
GCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCT1251420.1847443195129843No Hit
TGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA1250880.18466460052772196No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT1218350.17986226980441772No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG1218140.17983126797681573No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAG1190220.17570950118325118No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG1189580.17561501942294025No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTA1188700.1754851070025127TruSeq Adapter, Index 9 (97% over 35bp)
CGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGCCAAGGG1177140.17377853020689646No Hit
GCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCT1124380.16598969009126377No Hit
GCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGA1124320.16598083242623463No Hit
GTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCT1108910.1637058887912479No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA1102710.1627905967382357No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGA1073740.15851382080666107No Hit
CCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTG1054750.15571036982493505No Hit
GAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACT1047250.1546031616962913No Hit
GCCGGGCGCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCA1039010.153386709032288No Hit
CCGGGCGCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCAC1028860.15188828736485677No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCCGCTCGTGGGGGG1026230.151500026381079No Hit
GGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCG1006160.1485371374288283No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG1001080.14778718845636024No Hit
GGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGA996340.1470874329190574No Hit
CATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAA961830.14199279924979122No Hit
AAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCT925660.13665310351471854No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC925250.13659257613701936No Hit
GGCCAACCGAGGCTCCTTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGA913160.13480775663364558No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG907310.13394413429330343No Hit
GGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAG905160.1336267346297589No Hit
GGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGAT891130.13155551729044263No Hit
GCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG883570.13043945149676972No Hit
GGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATT870590.12852324329546358No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG869090.12830180166973482No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA865300.12774229249539348No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA842100.12431732868412211No Hit
CGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCT840660.1241047447234225No Hit
GCTCCTTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAG827640.12218263141209693No Hit
CGGCGCGCCGGGCTCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAG820840.12117876270879326No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG816920.12060006192688877No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAG807690.11923745778990452No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA797460.11772722590243441No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG796160.11753530982680284No Hit
GCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCT791910.11690789188723803No Hit
GGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTA790690.1167277860316453No Hit
GCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAA788330.11637938454049873No Hit
CTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGC787900.11631590460778982No Hit
GCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAG785660.11598521844670155No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT783010.11559400490791409No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC779870.11513045377138857No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT774400.11432292997623104No Hit
CGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTAC764660.112885035686499No Hit
GGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAG761030.11234914695223543No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT759200.11207898816884634No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC750290.11076362491201756No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGA745980.1101273493074236No Hit
TCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGT742260.10957817407561629No Hit
GGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTC741450.10945859559772277No Hit
CGGGCGCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACC733750.10832186191898183No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA721830.10656213913319067No Hit
GCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGC720650.10638793838761738No Hit
CGGCCCCCGGCGCGCCGGGCTCGGGTCTTCCCGGAGTCGGGTTGCTTGGG718310.10604248945148052No Hit
CGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGG714690.10550807699472181No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA713360.10531173208657564No Hit
CTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTT700730.10344719359793955No Hit
CCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCT698930.10318146364706503No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC697110.10291278114118081No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC693330.10235474824434436No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG689110.10173175913729413No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTT685780.1012401587281763No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC684730.10108514959016617No Hit
CGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGAT681100.10054926085590259No Hit
GCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCT678480.1001624761496297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG176650.081.220611
AATCGGA183650.077.848072
GAGCACA5456950.055.781999
AGAGCAC5669900.053.7129028
CGCGTAA582200.051.4663771
AAGAGCA6098500.050.0189747
CCTGGCC117150.048.0561561
TCGGAAG6419650.047.4104583
TGCCGTC3463250.046.1257550-51
GCGTAAC657600.045.915862
CGGAAGA6691050.045.881654
CTCGTAT3485550.045.8749944-45
GATCGGA6626650.045.793411
CTCACCC470050.045.683852
GAAGAGC6674050.045.650356
GTATCTC3560250.045.562440-41
CTTGAAA3434000.045.45155362-63
TCGTATC3732350.045.30803738-39
GGCGCTT45050.044.9145552
TATGCCG3658850.044.8098748-49
Produced by FastQC (version 0.11.5)