FastQCFastQC Report
Tue 26 Nov 2019
HT27GBGXC_n01_PACT_MOCK.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT27GBGXC_n01_PACT_MOCK.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64773848
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGCGATATCAGCCGAAGAAGGACGCGGTAAGCTGCGATAAGCTACGG2079470.32103542775473215No Hit
GTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATGCAA2040590.31503300529559397No Hit
CCTCGATCGATTAGTACCAGTCAGCTCCAAACCTCACGGCTCTTCCACAC1709810.2639660994048092No Hit
CTAGGAGATACACCTGTACCCATGCCGAACACAGAAGTTAAGCCCTAGAA1160770.17920349583060127No Hit
AGGAGATACACCTGTACCCATGCCGAACACAGAAGTTAAGCCCTAGAACG1013670.1564937133270205No Hit
GGATGCCTTGGCGATATCAGCCGAAGAAGGACGCGGTAAGCTGCGATAAG916010.14141664086407219No Hit
CGGATGCCTTGGCTCACGGAGGCGATGAAGGACGTGATAAGCTGCGATAA899060.13879984403582138No Hit
TGATAAGCTGCGATAAGCCTTGGGTAGGTGCAAATAACCTTTGATCCAAG884280.13651805895490415No Hit
CTGCGATAAGCCTTGGGTAGGTGCAAATAACCTTTGATCCAAGGATTTCC866610.13379010615518783No Hit
GTCGATATCAGTATTTTGGCTTGGAGGGTGGTCCCCCCTGCTTCCCACAA852480.13160867021517697No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG797040.12304966041233184No Hit
CCCGGACGTAAGGGCCGTGCTGATTTGACGTCATCCCCACCTTCCTCACA751590.11603293971357083No Hit
CCTGGACCGATTCTCTGCGCCTCATATTGCTATGAGGACCCCTTATCCCG726080.11209462189122994No Hit
GGCGGATGCCTTGGCTCACGGAGGCGATGAAGGACGTGATAAGCTGCGAT717750.11080860905469134No Hit
CTGGATTCTTCTCCTCTCGGGGACGGACCTTAGCACCCGCCCCCTTACTG714500.11030686335015948No Hit
CGCGAAACCAAGTGATCTACACTTGCCCAGGTTGAAGTCCGGGTAACACC694300.1071883208173768No Hit
CTTCGTTCGCCTCACTACTACTGTAGTGATAACAGGTCTTCAACCTGTTA682580.10537894861518804No Hit
GCGACTTCCATATCTCACAGGGGGCAACCCCCAACTACTTCCGGCGTTCT664520.10259078633092789No Hit
CGACGATATGCCTTGGGTAGCTGTAAGTAAGCGATGATCCAGGGATTTCC664370.10256762883687257No Hit
CGCGTATCCAACCTTCCCATTACTGTGGGATAACCTGCCGAAAGGCAGAC659210.10177101104136967No Hit
CTCTTCCTAAAGGTACTAAGATGTTTCAGTTCCCTTCGTTCGCCTCACTA658180.10161199624885647No Hit
CTCATATCGACGGGGTGGTTTGGCACCTCGATGTCGGCTCGTCACATCCT652910.10079839629104634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATC241700.074.0666962
TCGATCG259400.069.8723
CGATCGA275250.066.624444
CGATTAG292950.063.0025338
TGATCCT408100.059.6469764
ATCGATT334100.055.5294766
GCGTACG74500.054.3196332
GATCGAT385800.049.8609625
TCCTGGC499200.049.4645357
GATTAGT388550.047.562329
CGTACGG87800.046.036243
CGCCCTC28250.045.7432021
AGGATCC52400.045.502713
GTCCCAC64050.042.712218
CTGGCTC572500.042.368119
TAGGAGA271700.042.253292
CCTGGCT592250.041.027428
CGGCCTC19100.037.3107531
GGATCCA69750.036.226684
CGCGGGG11150.035.3654631
Produced by FastQC (version 0.11.5)