FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32057872
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA921640.28749256968771975No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT331470.10339738083675672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG138500.044.277311
ACGTCTG140250.042.85756315
CACACGT150250.040.72629512
ACACGTC152650.040.08611313
CACGTCT150400.039.84894614
AGCACAC170950.037.16053410
CGTCTGA164500.036.39327216
TCGGAAG186500.033.7314873
CTGAACT179850.033.63335819
AGAGCAC193800.032.5446748
CAGTCAC155050.031.54920427
GTCACTC97950.031.09433229
CCAGTCA163700.030.69149226
AGTCACT115750.029.63502928
ACTCGGC68150.029.15570832
CTCCAGT184750.028.94994724
ACTCTAG195550.028.8804325
GTACTCT162250.028.864363
TGTACTC157550.028.8327052
TACTCTA170550.028.319134
Produced by FastQC (version 0.11.5)