FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-TGA1-Endo-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-TGA1-Endo-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21427725
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1088760.5081080702687756No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC903670.4217293249750032TruSeq Adapter, Index 10 (97% over 36bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA449460.20975628537327223No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA366220.17090941758866143No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224210.10463546643425749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG143800.055.7103246
GTATGCC146150.055.00687845
CGTATGC144150.054.89600444
ACGTCTG183550.051.69544215
GCACACG184500.051.50261711
CTCGTAT145200.050.57043542
ATGCCGT154900.050.54314847
TCACTCG185650.050.4686230
TCGTATG154400.049.75560843
CACACGT194500.048.73063712
AGCACAC197000.048.4657510
ACACGTC197000.048.18340713
CACGTCT199050.047.70442214
GTCACTC198150.047.3740829
AGTCACT203150.046.2771228
CAGTCAC206950.045.49534627
TGCCGTC169350.045.1558448
CCAGTCA208950.045.07684726
CACTCGA209400.044.82807531
TCCAGTC212250.044.4910225
Produced by FastQC (version 0.11.5)