FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-TGA1-Endo-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-TGA1-Endo-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22117827
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT329420.14893868190577672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA137250.027.4171454
ACTCTAG170600.026.8384725
GTACTCT148750.025.3209173
TGTACTC155150.024.2312412
CTAGTTG154550.024.140688
CTCTAGT156800.023.9097736
TCTAGTT158950.023.8909517
TTGTACT156200.023.710011
GGACACC154750.022.7303229
ACCTACT114250.022.4240633
GACACCT158200.022.2786730
ACCTCTA165200.022.22533817
ACACCTA116750.021.8543531
GTTACCT169400.021.69441814
CTAATGC170700.021.44771821
TGGACAC173700.020.53267328
TACCTCT180350.020.4747816
TAGTTGT189700.020.460949
GCACACG43850.020.4328411
TGTTACC185550.019.881813
Produced by FastQC (version 0.11.5)