FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-TGA1-Endo-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-TGA1-Endo-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22277617
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT593610.2664602771472371No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT245230.11007909867558993No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC241960.10861125765830339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA180650.036.139214
GTACTCT188000.034.5957453
TTGTACT189100.034.153851
ACTCTAG247700.033.8469245
TGTACTC194750.033.342592
CTCTAGT203350.032.0537726
TCTAGTT205250.031.8023597
TATGCCG44000.031.18045446
CTAGTTG206950.031.1183138
GTATGCC44000.030.70347845
GTTACCT217250.029.49850514
GGACACC210050.029.46014829
CGTATGC46700.029.30305144
ACCTACT160150.029.26263233
GCACACG51650.029.2055111
ACACCTA160400.029.1522231
ACCTCTA221600.028.9195817
CTAATGC219950.028.85041821
GACACCT216350.028.61807830
TGGACAC223250.027.81234228
Produced by FastQC (version 0.11.5)