FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-EV-Endo-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-EV-Endo-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22520575
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT684470.3039309609101899No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA295130.13104905181151014No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC242790.10780808216486479TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG54250.034.64369246
GTATGCC56100.034.12533645
CGTATGC55300.034.112744
TTGTACT207950.033.313211
TACTCTA214200.032.7986954
GCACACG65600.032.06475411
ACTCTAG249350.031.6708935
TGTACTC224100.031.2091732
GTACTCT226500.031.0793933
ACGTCTG68650.030.69238315
CTCTAGT238300.029.6580686
TCTAGTT237450.029.6118167
ATGCCGT69000.027.54224647
CTAGTTG254550.027.4576428
CACACGT79600.026.55819312
CTCGTAT71000.026.42158742
TCGTATG74400.025.91977143
AGCACAC82400.025.90949610
ACACGTC82550.025.69396813
ACACCTA195550.025.6840231
Produced by FastQC (version 0.11.5)