FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-EV-Endo-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-EV-Endo-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22634741
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT746930.3299927310853701No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA286940.12676972977070955No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA243870.10774145814171232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT222200.035.492161
TACTCTA232250.034.51394
TGTACTC234050.034.203452
ACTCTAG270000.033.0982635
GTACTCT244400.032.9554983
CTCTAGT255400.031.5640776
TCTAGTT262800.030.7777677
CTAGTTG272600.029.2350278
GTTACCT290150.027.08149314
ACCTACT205900.026.97640233
ACACCTA206000.026.94661531
ACCTCTA293400.026.75776717
GGACACC283400.026.46643329
TAGTTGT303450.026.3782129
GACACCT287400.026.18303330
CTAATGC298150.026.1203421
GTATGCC45450.025.7196247
TATGCCG45150.025.65790748
TACCTCT306800.025.61178216
TGGACAC295400.025.56901628
Produced by FastQC (version 0.11.5)