FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-EV-Endo-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-EV-Endo-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19523718
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT950760.4869769169991085No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA402840.2063336501787211No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA359750.1842630589112176No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT205240.10512341962734763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT251800.041.4135861
TGTACTC269850.038.9156842
TACTCTA272350.038.7771574
GTACTCT278200.037.8735733
TCTAGTT296050.035.4546137
ACTCTAG342300.035.3730745
CTCTAGT308350.034.238736
TAGTTGT332900.031.3088159
CTAGTTG336000.031.0303738
GTTACCT338450.030.39405314
ACACCTA241750.030.0122531
ACCTACT243200.029.67485633
CTAATGC345600.029.50214821
TGTTACC350950.029.42142113
TGGACAC343650.029.1999128
TACCTCT354100.029.07073616
ACCTCTA358100.028.75578317
TAATGCT358650.028.48700722
TCTAATG359700.028.4332620
ATGCTGG363650.027.95102124
Produced by FastQC (version 0.11.5)