FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-EV-Col-0-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-EV-Col-0-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33609576
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT500510.14891886764653026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA204450.026.9144824
ACTCTAG270500.026.2954675
TGTACTC225950.024.2604432
CTAGTTG228550.023.7361538
TTGTACT228900.023.6572951
GTACTCT234100.023.475683
TCTAGTT238600.023.2790417
CTCTAGT251850.022.0437626
GGACACC236350.021.59109729
ACCTCTA254250.021.02147117
ACCTACT177750.020.95062633
CTAATGC257800.020.65041421
GTTACCT263750.020.42348914
GACACCT248700.020.4061230
TACCTCT275350.019.58856416
ATGCTGG268550.019.57603324
ACACCTA194050.019.35326831
TTACCTC285300.018.97895415
TGGACAC271800.018.96822528
TAGTTGT292100.018.9314889
Produced by FastQC (version 0.11.5)