FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-EV-Col-0-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-EV-Col-0-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26917886
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT645060.23963991823132022No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA280130.10406835068697444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA224150.032.9979634
ACTCTAG273200.031.3402845
TGTACTC236400.031.243332
GTACTCT241550.030.620853
TTGTACT237850.030.538051
TCTAGTT261750.028.2134367
CTAGTTG263550.027.635528
CTCTAGT269300.027.5567196
GGACACC279050.025.03555729
ACCTACT200350.024.87629533
ACCTCTA293250.024.65879417
GTTACCT293900.024.65171414
CTAATGC290450.024.59556621
GACACCT286600.024.400130
TACCTCT302350.023.93995716
TAGTTGT315050.023.6290689
TGGACAC299550.023.55595828
ATGCTGG301750.023.4541624
TTACCTC311200.023.3264115
ACACCTA216000.023.10670931
Produced by FastQC (version 0.11.5)