FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-EV-Col-0-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-EV-Col-0-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38945678
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT397090.10195996587862716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG260900.023.3458025
TACTCTA191750.022.96564
CTAGTTG209050.020.6763828
TCTAGTT230150.019.7998147
TGTACTC220950.019.5661532
GTACTCT223500.019.4995143
GGACACC210500.019.37083429
CTCTAGT237650.018.9425536
ACCTACT162000.018.60162733
ACCTCTA232300.018.56283817
TTGTACT231300.018.3425881
CTAATGC235400.018.08036621
GACACCT226550.017.98282230
ACACCTA176650.017.21767631
GTTACCT250900.016.99119414
ATGCTGG253350.016.6197224
TACCTCT262000.016.44521916
AGGACTG254400.016.27497962
TGGACAC253700.016.2517228
TTACCTC271000.015.98941515
Produced by FastQC (version 0.11.5)