FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-ABF3-Endo-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-ABF3-Endo-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21243990
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT851480.40080982903870693No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA358760.16887599739973516No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA264810.12465172502905528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT239300.038.2222981
ACTCCCG65700.037.34424632
GCACACG69350.036.43740511
TACTCTA255800.036.071484
TGTACTC257700.035.6831932
GTACTCT263900.034.9642373
ACGTCTG73650.034.7871315
ACTCTAG294400.034.4689835
CTCTAGT281550.033.0211876
TCTAGTT284800.032.5907487
CACACGT80050.031.83122412
CCCGAAT74500.031.05324635
ACACGTC83200.030.58400513
AGCACAC84800.030.37663710
CACGTCT84300.030.26753814
CTAGTTG307050.030.0009888
CACTCCC85250.028.82117731
CTCCCGA85650.028.72723833
ACCTACT232300.028.53623833
ACACCTA233350.028.49802831
Produced by FastQC (version 0.11.5)