FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-ABF3-Endo-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-ABF3-Endo-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27610416
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT417960.15137765399840408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG216200.027.0551475
TACTCTA172750.026.9907254
TGTACTC187800.024.8836942
GTACTCT188950.024.6580963
TTGTACT184450.024.5006921
CTCTAGT191900.024.4068586
TCTAGTT197050.024.0137627
CTAGTTG197650.023.2501358
GTATGCC48500.022.58667845
GGACACC194200.022.27663829
ACACCTA148150.021.94757831
ACCTACT146900.021.8956833
GTTACCT209600.021.74175514
GACACCT202000.021.57200630
CTAATGC210100.021.55701421
ACCTCTA213400.021.50232917
TATGCCG50700.021.33026946
CGTATGC53250.021.16342744
GCACACG57250.020.17429711
TGGACAC215950.020.09781628
Produced by FastQC (version 0.11.5)