FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-ABF3-Endo-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-ABF3-Endo-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24903547
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT594380.2386728284127558No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA251510.10099364560397761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA197300.031.3854184
TTGTACT196500.030.800631
ACTCTAG260550.030.6990495
TGTACTC203650.030.3551642
GTACTCT204800.030.235993
CTCTAGT217300.028.8029886
TCTAGTT219550.028.6786427
CTAGTTG227650.027.2585588
GTTACCT240100.025.55438814
ACACCTA170700.025.42496531
ACCTCTA242150.025.35245117
GGACACC233250.025.1796429
GACACCT236550.024.90195330
CTAATGC246650.024.63423521
ACCTACT175950.024.58622633
TAGTTGT262100.024.0495859
CGTATGC42250.023.60879544
TGTTACC261400.023.5392913
CACCTAG50600.023.51801331
TGGACAC251650.023.49156228
Produced by FastQC (version 0.11.5)