FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-ABF3-Col-0-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-ABF3-Col-0-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21556560
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT426510.19785624422449596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA147050.031.4938934
ACTCTAG196300.029.387965
GTACTCT158250.029.375543
TGTACTC158100.029.1598592
TTGTACT158900.028.6608411
GCACACG45650.027.37003511
TCTAGTT172300.027.2392067
CTAGTTG170000.027.175218
ACCTACT127550.025.40958633
CTCTAGT185200.025.3844976
GGACACC177500.024.84570329
CTAATGC182250.024.7936721
ACCTCTA185200.024.6823417
GTTACCT191450.024.004114
GACACCT185650.023.75481630
ACACCTA137300.023.6311231
TACCTCT194100.023.60452716
ATGCTGG192200.023.20038624
TGGACAC191700.023.15134628
TAGTTGT205450.022.9120549
Produced by FastQC (version 0.11.5)