FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-ABF3-Col-0-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-ABF3-Col-0-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25904007
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT346780.13387118062468095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA138150.028.4803374
GTACTCT152750.025.7810763
ACTCTAG213450.025.5998945
TCTAGTT164050.024.4494197
TGTACTC159550.024.4189172
CTAGTTG163300.024.1115328
TTGTACT163350.023.4653151
ACCTACT117350.023.35288233
CTAATGC165150.022.93140221
GTTACCT169650.022.67352514
ACCTCTA174100.022.1344117
GGACACC168100.021.94529529
ATGCTGG173100.021.87814324
TACCTCT179150.021.53000816
ACACCTA128750.021.28529531
CTCTAGT192600.021.0649416
TGGACAC179200.020.93756328
AGGACTG181650.020.63526362
GACACCT180100.020.61900530
TAGTTGT194000.020.4583119
Produced by FastQC (version 0.11.5)