FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-ABF3-Col-0-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-ABF3-Col-0-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24379045
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT389000.1595632642706062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA152150.029.5177424
GTACTCT163150.027.6562543
ACTCTAG213150.027.5571945
CTAGTTG164700.027.3063728
TGTACTC167950.026.5323622
TCTAGTT173200.026.3300087
TTGTACT168100.026.3005031
ACCTACT118300.025.53237233
CTAATGC175950.024.84608321
CTCTAGT188950.024.3987336
GGACACC173550.024.05997829
ACCTCTA184400.023.8783117
ACACCTA127900.023.75321231
ATGCTGG184500.023.42903724
GACACCT180650.023.24974630
GTTACCT189750.023.16788314
TACCTCT190700.023.16278316
TGGACAC183450.023.02867328
TAGTTGT201250.022.3992549
AGGACTG197000.022.15399262
Produced by FastQC (version 0.11.5)