FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-ABF2-plus.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-ABF2-plus.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19295400
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGC4904152.5416161364884897TruSeq Adapter, Index 10 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1597270.8277983353545404No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA597200.30950381956321193No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA590320.30593820288773493No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATGTCGTATGC539410.27955367600568015TruSeq Adapter, Index 10 (97% over 36bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG305490.15832270903946016No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCGCGTATGC293200.15195331529794665TruSeq Adapter, Index 10 (97% over 36bp)
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT291860.15125884925940897No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG279000.14459404832239808No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277330.14372855706541457No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA276120.14310146459777978No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA275330.1426920405899852No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC246610.12780766400281932No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG220600.11432776723986028No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTTTGC214570.1112026700664407TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGG211490.10960643469427947TruSeq Adapter, Index 10 (97% over 36bp)
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC198310.10277579112119986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1118100.067.0708411
ACGTCTG1118050.066.9333115
ACACGTC1122600.066.7896813
CACACGT1122600.066.783112
AGCACAC1125500.066.6177610
CACGTCT1126700.066.4562114
TCACTCG1112150.066.173830
ACTCGGC1112700.066.1283432
CACTCGG1115200.066.0524531
GTATGCC684300.065.99845
GTCACTC1119100.065.78194429
CGGCAAT1068550.065.7666735
CGTCTGA1138100.065.7543116
TATGCCG676000.065.7257646
AGTCACT1121750.065.6889428
CGTATGC674100.065.63116544
GAGCACA1143350.065.52289
CTCGGCA1119550.065.4752333
CAGTCAC1127250.065.359827
TCCAGTC1131650.065.2228725
Produced by FastQC (version 0.11.5)