FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-ABF2-Endo-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-ABF2-Endo-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22759643
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT583780.25649787213270436No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGC408880.17965132405635711TruSeq Adapter, Index 3 (97% over 36bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA240030.10546298990717913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC82650.045.90294645
TATGCCG83050.045.63931746
CGTATGC84500.044.93939244
GCACACG95950.043.55225411
ACGTCTG98400.042.57794615
CACACGT106500.039.3389312
ACACGTC108050.038.80742313
ATGCCGT95450.038.5735147
TCGTATG99100.038.1420743
AGCACAC111050.037.94548810
CACGTCT110850.037.79551314
CTCGTAT89300.037.74268742
GTCACTC121550.034.03608329
TGCCGTC110050.032.75621448
TACTCTA194450.032.511324
AGTCACT128000.032.29363628
TTGTACT195800.031.9831681
GAGCACA133650.031.7122769
CGTCTGA134750.031.32621816
TGTACTC202950.031.1840952
Produced by FastQC (version 0.11.5)