FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-ABF2-Endo-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-ABF2-Endo-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22698851
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTCGTATGC1252610.5518385049534005TruSeq Adapter, Index 6 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1117830.49246105012099517No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA454980.20044186377539552No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA386190.17013636505213414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG238850.056.915
GCACACG239650.056.7521211
CGTATGC189100.056.54231344
GTATGCC192450.055.88541845
TATGCCG192150.055.44396646
AGTCACG246350.054.42855528
CACACGT252000.053.8897412
ACACGTC252600.053.830913
AGCACAC254400.053.47553310
CACGTCT255150.053.3063914
TCGTATG198400.053.08042543
GTCACGA255050.052.51693729
CTCGTAT193000.051.55542442
ATGCCGT203450.051.0915247
CGTCTGA269800.050.30809816
CAGTCAC269150.049.96133827
GAGCACA276850.049.2151159
TCACGAC276950.048.60392830
CCAGTCA276200.048.5350826
TCCAGTC279500.048.1246225
Produced by FastQC (version 0.11.5)