FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-ABF2-Endo-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-ABF2-Endo-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24499848
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT539870.2203564691503392No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT247150.10087817687685245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA185550.031.769464
ACTCTAG255800.030.7217735
GTACTCT194950.030.3094393
TTGTACT194500.029.875761
TGTACTC201800.029.2458482
TCTAGTT211350.028.2350067
CTCTAGT214750.027.7432796
CTAGTTG217550.027.043948
ACACCTA160050.025.58582931
ACCTACT160050.025.43223433
GTTACCT229250.025.38980114
CTAATGC228700.025.12998821
GGACACC226450.024.71448929
ACCTCTA236600.024.64579817
TAGTTGT242300.024.6424739
GACACCT228950.024.55128530
TGGACAC237450.023.71702828
TGTTACC248650.023.53583113
TACCTCT252700.023.13087516
TCTAATG250100.023.04955320
Produced by FastQC (version 0.11.5)