FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-ABF2-Col-0-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-ABF2-Col-0-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31898491
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT345790.10840324703761064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG228600.025.2509885
TACTCTA159250.025.0587084
CTAGTTG162800.024.293118
GGACACC168100.022.0496829
GTACTCT183200.021.7253553
ACCTACT129050.021.34423333
TCTAGTT191950.021.333347
CTAATGC180450.021.2391321
ACCTCTA184500.021.13324717
TGTACTC188150.021.0794012
GACACCT179650.020.96297630
TTGTACT187750.020.6957131
CTCTAGT201700.020.131966
ATGCTGG192750.019.92001524
ACACCTA141300.019.69213931
AGGACTG195300.019.67667462
GTTACCT200100.019.31058114
GGACTGT200800.019.19024863
TGGACAC200100.018.82087928
TACCTCT206850.018.66368716
Produced by FastQC (version 0.11.5)