FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-ABF2-Col-0-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-ABF2-Col-0-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28834081
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT357050.12382915897336906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG212100.026.2246885
TACTCTA149900.026.0841314
CTAGTTG160350.024.2276388
GTACTCT168250.023.0936183
GGACACC159700.022.85873829
TCTAGTT177200.022.595567
TGTACTC172650.022.5861762
ACCTACT117300.022.37833833
CTAATGC175800.021.88063821
ACCTCTA178300.021.86833817
GACACCT172600.021.27170430
TTGTACT181250.021.0704151
CTCTAGT191250.020.9754756
AGGACTG184800.020.2266262
ACACCTA131800.020.18207231
GTTACCT192400.020.11990414
ATGCTGG188050.020.00847624
GGACTGT192850.019.43706363
TGGACAC191200.019.34909628
TACCTCT201950.019.32467516
Produced by FastQC (version 0.11.5)