FastQCFastQC Report
Wed 18 Dec 2019
HT23WBGXC_n01_1119-ABF2-Col-0-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT23WBGXC_n01_1119-ABF2-Col-0-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38160095
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG238600.021.1408985
TACTCTA149850.018.968264
CTAGTTG150500.018.8833688
TCTAGTT185550.016.467067
GTACTCT177850.016.1787743
GGACACC167000.015.67686729
ACCTACT123450.015.59331133
CTAATGC176700.015.54945821
TGTACTC187100.015.3601662
ACCTCTA184550.014.88806617
GCTGACT143350.014.8469021
CTAGCAG146800.014.71021658
GACACCT183450.014.40448330
GTTACCT193850.014.336122514
AGGACTG187450.014.32058562
CTCTAGT220600.014.1067626
ACACCTA139450.014.08032931
GGACTGT193800.013.90573863
TGGACAC195000.013.87455528
TTGTACT200750.013.5660681
Produced by FastQC (version 0.11.5)