FastQCFastQC Report
Tue 27 Oct 2020
HT237DRXX_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT237DRXX_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027975317
Sequences flagged as poor quality0
Sequence length91
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCATTCGTAAACAAACCCT19752780.19215227908045188No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11798460.11477376747169506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT52296700.076.512221
AGCAGTG56378200.070.915492
GCAGTGG62322500.064.109763
CAGTGGT64260000.062.1331674
AGTGGTA66838450.059.771785
TGGTATC68397450.058.3257337
GTGGTAT68930150.057.9118736
GGTATCA70011450.057.0802848
GTATCAA72170550.055.4475949
CATGGGG45152350.031.52216124-25
AGAGTAC69789250.031.24887818-19
GCAGAGT70386450.031.19674516-17
CAACGCA71149100.031.12789712-13
TACATGG69143200.030.9928122-23
ATCAACG71833100.030.81058710-11
ACGCAGA72260700.030.60831514-15
AGTACAT74716600.028.9446520-21
ACATGGG67679050.028.50224924-25
CAGAGTA70021300.028.38935918-19
TCAACGC71407550.028.0718312-13
Produced by FastQC (version 0.11.5)