FastQCFastQC Report
Tue 27 Oct 2020
HT237DRXX_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT237DRXX_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027975317
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAACCCT585037165.691159605926608No Hit
TTTCTGTC542061235.2730957741468805No Hit
AGCGAAAG523121215.088849910585937No Hit
GTCTCCTT489868004.765367338095337No Hit
AAGATCAT487268004.7400749020124575No Hit
CGAGGCTC485331774.721239527582937No Hit
CAGATGGG469238794.564689270647147No Hit
AGTGGAAC469238164.564683142095327No Hit
CTGCGATG464153264.515217946619238No Hit
CCTGAAGG461307804.487537710012934No Hit
GTCCGGTC443908934.318283937940117No Hit
TCACATCA435780264.239209373934803Illumina PCR Primer Index 1 (100% over 8bp)
TGTAAGGT422015454.105307228889426No Hit
TCTTAAAG402590903.9163479253072375No Hit
GCATCTCC402473763.915208403783103No Hit
AACGTCAA395655223.8488786010413514No Hit
GTCCTTCT380739543.7037809537210897No Hit
TGATCTCA380148283.698029259198643No Hit
AAGACGGA356625893.4692067416634282No Hit
TCCGGAAG348850543.393569225164576No Hit
CAGCATCA318003053.0934891601098626No Hit
GTAATCTT272577632.651597032460654No Hit
AGTTCGGC244823372.3816074758923422No Hit
GGGGGGGG89840060.8739515289353976No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)