FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282038653
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11876470.4210937002312233No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCTC22026500.0141.541188
CCAATGT6427200.0140.09718
CAATGTT14908750.0139.648869
TCCTGCA7384650.0139.495838
CCTGCAC15665850.0139.364919
CACCGAC4886950.0137.326289
CCACCGA2378250.0136.944958
TCAATGT3687550.0135.787778
CCAAAAG6386900.0135.76261
TAAAAGC11100050.0134.963382
TCCAATG2579550.0133.986917
CTTCTCA42840400.0133.24489
CTAAAAG4605900.0133.243271
GGCCTTC1691300.0133.229026
GCCAATG1176050.0133.045937
GATCTGC3315600.0132.197989
TCCCTTC1675700.0131.782626
TACTTCT2230750.0131.450337
ATCTTCT2242350.0131.184167
TGCCTTC1260900.0130.316546
Produced by FastQC (version 0.11.5)