FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l02_n02_bc04_g99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l02_n02_bc04_g99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28158353
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCAC27673950.0144.915279
ACCTGCA6831850.0144.91068
CCCTGCA8878250.0144.901118
GCCTGCA4794750.0144.872428
TCCTGCA7251150.0144.826428
CACCTGC2146650.0144.253777
AACCTGC1656050.0144.209787
TCCCTGC2485700.0144.191337
TACCTGC1930550.0144.189777
ACCCTGC2155050.0144.179297
CCCCTGC2645200.0144.168447
ATCCCTG574200.0144.124256
CTCCTGC2091800.0144.116557
CCGCCTG426400.0144.111336
ACGCCTG341150.0144.10296
GCTCCTG414650.0144.068776
GACCCTG393300.0144.055346
GTCCTGC1383350.0144.05537
CCCCCTG750350.0144.038916
CTACCTG505900.0144.020986
Produced by FastQC (version 0.11.5)